Background/context:

Leifsonia (formerly Clavibacter) xyli subsp. xyli (Lxx) is the causal agent of ratoon stunting disease, and one of the most important pathogens of sugarcane in Australia.

 Aim:

The aim of this project was to sequence the DNA flanking the transposon insertions from 750 transposon insertion mutants, to map where the transposons have inserted into the Leifsonia xyli subsp. xyli (Lxx) genome, to make DNA arrays that represent every gene in the Lxx genome, to determine if all the genes that are regulated both positively and negatively during interaction of Lxx with sugarcane, to determine whether there are different expression patterns in the Lxx genome during interaction with an Lxx -susceptible compared with a resistant sugarcane cultivar, and to characterise genes differentially expressed during these interactions.

Major Outcomes:

The full sequencing of Leifsonia xyli subsp. Xyli genome was completed and is publicly available to assist future research in this area.

For more information contact:

Dr Stevens Brumbley
Bureau of Sugar Experiment Stations
Phone: +61 (0)7 3331 3370
Email: sbrumbley@bses.org.au